Comparison of Different Ways of Chromatographic Spots Visualization in Determination of Antyhistamine Substances

Importance of compounds investigated (loratadine, desloratadine, cetirizine dihydrochloride, levocetirizinedihydrochloride) in contemporary pharmacy causes necessity of finding the fast and easy method for their identification. The aim of this work is to find the best way of visualization of chromatographic spots in qualitative determination of compounds investigated during analysis by thin layer chromatography. The standard solutions of compounds investigated were prepared in ethanol or methanol. The different mobile phases for particular compounds were used. There were also used many ways of spot visualization i.e. exposure to UV light, dipping in solution of visualizing agents, visualization in iodine vapour and photo catalytic visualization. The best visualization effect gave exposure to UV light and dipping in solution of bromophenol blue in the case of loratadine and desloratadine, respectively; and visualization in iodine vapour in the case of cetirizine and levocetirizine. Using photo catalytic degradation had no use in the case all of compounds investigated.